SUPLEMENTASI KELAPA KOPYOR TERHADAP AKTIVITAS SUPEROKSIDA DISMUTASE DAN PATOLOGI ANATOMI HATI TIKUS AKIBAT PERLAKUAN PARASETAMOL

Akhmad Endang Zainal Hasan, Hasim Hasim, Agus Setiyono, Sekar Winahyu Ariadini

Abstract


This research aimed at determining of the effects of the induction of paracetamol
and kopyor coconut on the activity of superoxide dismutase (SOD) and pathologic
anatomy of rats liver. Previous research shows that paracetamol caused negative effectto the body while kopyor coconut has the ability to improve human health, related to itscapacity to prevent oxidative damage of human body. In this research, the positiveeffect of kopyor is approached through measurement of body weight and the activity ofthe total SOD enzyme of animals tested, which are induced by paracetamol and fed withkopyor. Rats administered with temulawak were positive control. The result showedthat paracetamol treatment using toxic dose was found to reduce body weight of testedanimals. Meanwhile, both kopyor and temulawak supplements were able to counter thenegative effect of paracetamol, indicated by the increase in the body weight of testedanimals. Other results showed that treatments with temulawak and 5 times-dose ofkopyor supplement demonstrated a significantly high total SOD activity in the liver ascompared to negative control. Furthermore, pathologic observation of the testedanimals liver showed that 5 time-dose of kopyor treatment was confirmed to improve the recovery of the liver caused by negative effect of paracetamol, better than the negative control.

Key words: kopyor coconut, superoxide dismutase, free radical, pathologic


Keywords


kopyor coconut, superoxide dismutase, free radical, pathologic

References


Asikin, N. 2001. Antioksidan endogen dan penilaian status antioksidan. Makalah dalam kursus penyegaran dan pelatihan 2001. Radikal Bebas dan Antioksidan:Dasar, Aplikasi, dan Pemanfaatan Bahan Alam. Bagian Biokimia, Fakultas Kedokteran Universitas Indonesia. Jakarta. Hlm 1-6.

Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal protein-dye binding. Analyt Biochem 72:248-252

Dinu, D., Nechifor, M.T., Movileanu, L. 2005. Ethanol-induced alterations of the
antioxidant defense system in rat kidney. J Biochem Mol Toxicology 19: 386-395.

Fridovich, I. 1986. Superoxide dismutase. Advance in Enzymology and Related
Areas of Molecular Biology 58 :61-97

Gan, S. et al.1980. Farmakologi danTerapi edisi ke-2. Jakarta: UI Press.


Gitawati, R. 1995. Radikal bebas: sifat dan peranan dalam menimbulkan
kerusakan atau kematian sel. Cermin Dunia Kedokteran 102:33-36.

Gupta, A K., Chitme, H. Dass, S.K., Misra, N. 2006. Hepatoprotective
activity of Rauwolfiaserpentina rhizome in paracetamol intoxicated rats. J.
Pharmacol Toxixol. Methods 1: 82-88.

Hadi, S., Ediyanto, S.P., Djatmiko, W., dan Fuad, A. 1985. Pengaruh pemberian kurkuminoid (C. Domestica, Val) terhadap kadar kolesterol HDL serum tikus putih (Rattus norvegicus). Prosiding Simposium Nasional Temulawak. Universitas Padjajaran. Bandung. Hlm 170-177.

Halliwell, B., Gutteridge, J.M.C. 1989. Free Radical in Biology and Medicine
3 rd edition. New York: Oxford University Press.

Hasan, A.E.Z., Ambarsari, L., Artika, I.M., Julistiono H dan Tarunasari D. 2013. Induction resistance of Candida sp. Y 390 to ethanol stress by kopyor coconut


Full Text: PDF

DOI: 10.33751/jf.v3i1.172

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 FITOFARMAKA

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.