EXTRACTION OF ROBUSTA COFFEE BEANS (Coffea canephora) FROM WONOSOBO BY ULTRASONIC WAVES AND ANTICANCER TESTS
Abstract
Wonosobo Robusta coffee beans are one of the natural source ingredients that potentially could be used as ananticancer. It contains polyphenolic compounds such as chlorogenic acid, flavonoids, and hydroxy hydroquinone. Thisresearch aims to determine the optimum conditions for the extraction temperature and time of the Ultrasonic-Assisted Extraction (UAE) method on the extract yield and to determine the anticancer activity. Extraction optimizations wereanalyzed using Response Surface Methodology (RSM) with Central Composite Design (CCD) experimental design on atemperature factor of 40, 50, 60 ºC and extraction times of 10, 30 and 50 minutes. Determination of anticancer activitywas conducted using the Methyl Thiazole Tetrazolium (MTT) Assay method against MCF-7 cells. The optimization results indicate that the temperature of 52.74 ºC and time of 29,53 min are the optimum conditions for extraction with an extract yield of 9,16%. The anticancer activity test result at a concentration of 400 µg/mL is 83,25% so it has the potential to be used as an anticancer.
Keywords
References
Abdeltaif, S. A., Sirelkhatim, K. A., & Hassan, A. B. (2018). Estimation of phenolic and flavonoid compounds and antioxidant activity of spent coffee and black tea (Processing) waste for potential recovery and reuse in Sudan. Recycling, 3(2). https://doi.org/10.3390/recycling3020027
Afriliana, A. (2018). Teknologi Pengolahan Kopi Terkini. Deepublish. https://books.google.co.id/books?id=RFlVDwAAQBAJ
Aslantürk, Ö. S. (2018). In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. Genotoxicity - A Predictable Risk to Our Actual World, 118. https://doi.org/10.5772/intechopen.71923
Banožić, M., Banjari, I., Jakovljević, M., Šubarić, D., Tomas, S., Babić, J., & Jokić, S. (2019). Optimization of ultrasound-assisted extraction of some bioactive compounds from tobacco waste. Molecules, 24(8). https://doi.org/10.3390/molecules24081611
Bauer, D., Abreu, J., Jordão, N., da Rosa, J. S., Freitas-Silva, O., & Teodoro, A. (2018). Effect of roasting levels and drying process of coffea canephora on the quality of bioactive compounds and cytotoxicity. International Journal of Molecular Sciences, 19(11). https://doi.org/10.3390/ijms19113407
Bicho, N. C., Leitão, A. E., Ramalho, J. C., De Alvarenga, N. B., & Lidon, F. C. (2013). Identification of chemical clusters discriminators of Arabica and Robusta green coffee. International Journal of Food Properties, 16(4), 895904. https://doi.org/10.1080/10942912.2011.573114
Bravo, J., Arbillaga, L., de Peña, M. P., & Cid, C. (2013). Antioxidant and genoprotective effects of spent coffee extracts in human cells. Food and Chemical Toxicology : An International Journal Published for the British Industrial Biological Research Association, 60, 397403. https://doi.org/10.1016/j.fct.2013.08.002
Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68(6), 394424. https://doi.org/https://doi.org/10.3322/caac.21492
Damiani, R. M., Moura, D. J., Viau, C. M., Caceres, R. A., Henriques, J. A. P., & Saffi, J. (2016). Pathways of cardiac toxicity: comparison between chemotherapeutic drugs doxorubicin and mitoxantrone. Archives of Toxicology, 90(9), 20632076. https://doi.org/10.1007/s00204-016-1759-y
Dewatisari, W. F., Rumiyanti, L., & Rakhmawati, I. (2018). Rendemen dan Skrining Fitokimia pada Ekstrak Daun Sanseviera sp. Jurnal Penelitian Pertanian Terapan, 17(3), 197. https://doi.org/10.25181/jppt.v17i3.336
Fajrin, J., & Marchelina, N. (2017). Aplikasi Metode Eksperimen Response. 13(2), 7990.
Goebel H., Gustavo Ariel Rostagno, Maurico MartÃnez, J., da Fonseca Machado, A. P., & Garcia Mendoza, M. del P. (2016). Ultrasound-assisted extraction of antioxidants present in roasted coffee beans (Coffea Arábica L.). XXIV Congresso de Iniciação CientÃfica Da Unicamp, I, 50813. https://doi.org/10.19146/pibic-2016-50813
Górecki, M., & Hallmann, E. (2020). The antioxidant content of coffee and its in vitro activity as an effect of its production method and roasting and brewing time. Antioxidants, 9(4). https://doi.org/10.3390/antiox9040308
Gouthamchandra, K., Sudeep, H. V, Venkatesh, B. J., & Shyam Prasad, K. (2017). Chlorogenic acid complex (CGA7), standardized extract from green coffee beans exerts anticancer effects against cultured human colon cancer HCT-116 cells. Food Science and Human Wellness, 6(3), 147153. https://doi.org/https://doi.org/10.1016/j.fshw.2017.06.001
Haile, M., & Kang, W. H. (2019). Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation, 5(1). https://doi.org/10.3390/fermentation5010029
Hara, K., Haranishi, Y., Kataoka, K., Takahashi, Y., Terada, T., Nakamura, M., & Sata, T. (2014). Chlorogenic acid administered intrathecally alleviates mechanical and cold hyperalgesia in a rat neuropathic pain model. European Journal of Pharmacology, 723, 459464. https://doi.org/https://doi.org/10.1016/j.ejphar.2013.10.046
Kristiningrum, N., Cahyanti, Y. N., Wulandari, L., Farmasi, F., & Jember, U. (2016). Determination of Total Phenolic Content and Antioxidant Activity in Methanolic Extract of Robusta and Arabica Coffee Leaves. Proceeding ICMHS, 9699.
Król, K., Gantner, M., Tatarak, A., & Hallmann, E. (2020). The content of polyphenols in coffee beans as roasting, origin and storage effect. European Food Research and Technology, 246(1), 3339. https://doi.org/10.1007/s00217-019-03388-9
Kunarto, B., Sutardi, S., Supriyanto, S., & Anwar, C. (2019). Optimasi Ekstraksi Berbantu Gelombang Ultrasonik pada Biji Melinjo Kerikil (Gnetum gnemon L., ‘Kerikil) Menggunakan Response Surface Methodology. Jurnal Aplikasi Teknologi Pangan, 8(3), 104. https://doi.org/10.17728/jatp.5122
Lee, D. H., Jeong, I. J., & Kim, K. J. (2018). A desirability function method for optimizing mean and variability of multiple responses using a posterior preference articulation approach. Quality and Reliability Engineering International, 34(3), 360376. https://doi.org/10.1002/qre.2258
Li, H., Jin, S.-Y., Son, H.-J., Seo, J. H., & Jeong, G.-B. (2013). Caffeine-induced endothelial cell death and the inhibition of angiogenesis. Anatomy & Cell Biology, 46(1), 57. https://doi.org/10.5115/acb.2013.46.1.57
López-GarcÃa, J., Lehocký, M., HumpolÃÄek, P., & Sáha, P. (2014). HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation. Journal of Functional Biomaterials, 5(2), 4357. https://doi.org/10.3390/jfb5020043
Mikami, Y., & Yamazawa, T. (2015). Chlorogenic acid, a polyphenol in coffee, protects neurons against glutamate neurotoxicity. Life Sciences, 139, 6974. https://doi.org/https://doi.org/10.1016/j.lfs.2015.08.005
Minarni, Artika, I. M., Julistiono, H., Bermawie, N., Riyanti, E. I., Hasim, & Hasan, A. E. Z. (2017). Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Asian Pacific Journal of Tropical Medicine, 10(6), 566571. https://doi.org/10.1016/j.apjtm.2017.06.004
Ministry of Health of the Republic of Indonesia. (2015). INFODATIN: Situasi Penyakit Kanker. https://pusdatin.kemkes.go.id/article/view/15090700004/situasi-penyakit-kanker.html
Montgomery, D. C. (2013). Design and Analysis of Experiments Eighth Edition. Arizona State University. In Copyright (Vol. 2009, Issue 2005).
Odabaş, H. İ., & Koca, I. (2016). Application of response surface methodology for optimizing the recovery of phenolic compounds from hazelnut skin using different extraction methods. Industrial Crops and Products, 91, 114124. https://doi.org/https://doi.org/10.1016/j.indcrop.2016.05.033
Rao, S., & Nadumane, V. K. (2016). Evaluation of the anticancer potential of coffee beans: An in vitro study. Indian Journal of Traditional Knowledge, 15(2), 266271.
Risdian, C., Mozef, T., & Lotulung, P. D. . (2014). -. In Isolasi Siklokomunol dari Daun Sukun Artocarpus altilis (PARKINSON) FOSBERG serta Aktivitasnya sebagai Antikanker (Vol. 16, pp. 8286).
Safdar, M. N., Kausar, T., & Nadeem, M. (2017). Comparison of Ultrasound and Maceration Techniques for the Extraction of Polyphenols from the Mango Peel. Journal of Food Processing and Preservation, 41(4), e13028. https://doi.org/https://doi.org/10.1111/jfpp.13028
Sholihah, M. (2017). Aplikasi Gelombang Ultrasonik untuk Meningkatkan Rendemen Ekstraksi dan Efektivitas Antioksi dan Kulit Manggis. Jurnal Keteknikan Pertanian, 5(2), 161168.
Syakfanaya, A. M., Saputri, F. C., & Munim, A. (2019). Simultaneously extraction of caffeine and chlorogenic acid from Coffea canephora bean using natural deep eutectic solvent-based ultrasonic assisted extraction. Pharmacognosy Journal, 11(2), 267271. https://doi.org/10.5530/pj.2019.11.41
Utami, N. F., Nhestricia, N., & Maryanti, S. (2018). Antioxidant Activity Test from Robusta Coffee Seeds ( Coffea canephora P.) Based On High Flat Ecology Differences in Java Island. Fitofarmaka, 8(1), 6065.
DOI: 10.33751/jf.v12i1.4933


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