1. Home
  2. Vol 7, No 2 (2024)
  3. Setiawan

The Effectiveness of Beetroot and Apple Juice Combination on Physical Endurance and Hemoglobin Levels in Sprague Dawley Rats

Deni Setiawan, Moerfiah Moerfiah

Abstract


Physical performance in humans is closely linked to hemoglobin (Hb) concentration in the blood, making Hb levels a key parameter in evaluating fatigue and stimulant effects. This study aimed to assess the influence of beetroot and apple juice on endurance capacity and hemoglobin levels in white rats (Sprague Dawley). Endurance testing was conducted using a rotarod apparatus, while Hb levels were measured with the Sahli method using a hemometer. A total of 25 rats were randomly assigned into five groups (n = 5 per group): beetroot–apple (1:1) [2.8 g:0.63 g/200 g BW], beetroot–apple (2:1) [5.6 g:0.63 g/200 g BW], beetroot–apple (1:2) [2.8 g:1.26 g/200 g BW], negative control (CMC-Na 0.5%), and positive control (caffeine 20 mg/200 g BW]. Treatments were administered once daily. The observed variables were the duration of endurance on the rotating rotarod stick and Hb levels post-intervention. The findings demonstrated that the combination of beetroot and apple juice significantly enhanced both physical endurance and Hb levels in white rats. Among the treatment groups, the beetroot–apple ratio of 2:1 (5.6 g:0.63 g/200 g BW) yielded the most effective improvement in endurance performance and hemoglobin concentration compared to other tested formulations.

Keywords


beetroot; apple Juice; Hb Level; stimulant

References


Allenafina O. Tadete, Nancy S. H. Malond2, Anita. Basuki. (2013). Hubungan Antara Asupan Zat Besi, Protein Dan Vitamin C Dengan Kejadian Anemia Pada Anak Sekolah Dasar Di Kelurahan Bunaken Kecamatan Bunaken Kepulauan Kota Manado. Universitas Sam Ratulangi Manado

Bahri, S., Sigit, J.I., Ditia Y. (2009). Kadar Asam Laktat Hasil Metabolisme Anaerob Pada Atlet. Jurnal IPTEK Olahraga. 11 (1).

Bloom. (2001). The Health and Poverty of Nations : From Theory to Practice, School of Public Health, harvard University, Boston and dept. of Economics, Queens University, Belfast

Citra, H.U. (2016). Pengaruh Pemberian Ekstrak Etanol Umbi Bit Beta vulgaris L. Terhadap Peningkatan Stamina Mencit Putih Jantan. Skripsi, Universitas Andalas. Davis., J.M. Zhao., Z. Stock., S.H. Mehl.,

A.K. Buggy., J. dan Hand., A. G. (2002). Central Nervous System Effects of Vaffein and Adenosine on fatigue. Am J PhysiolRegul inegr Comp Physiol 284. University of South Carolina, Columbia, South Corolina 29208.

Giknis, Mary L.A and Clifford, C.B., (2006). Clinical Laboratory parameters for Crl:cd (SD) Rats, Charles River Laboratories Wilmington

Hanifan F, Ruhana A, Hidayati DYN. (2016). Pengaruh Substitusi Sari Umbi bit (Beta vulgaris L.) tehadap kadar kalium, Pigmen Betalain, dan Mutu Organoleptik Permen Jeli. Majalah Kesehatan FKUB. 3(1).

Herwana, E., Pudjiadi,. L.L., Wahab, R., Nugroho, D., Hendrata, T., Setiabudy, R. (2005). Efek Pemberian Minuman Stimulan Terhadap Kelelahan Pada Tikus. Universa Medicana. 24 (1).

Kumar, Yashwant. (2015). Beetroot : A Super food. Internaional Journal of Engineering Studies and Technical Approach. 1 (3).

Mulyawati, Yenni. (2003). Perbandingan Efek Suplementasi Tablet Tambahan Darah Dengan Dan Tanpa Vitamin C Terhadap Kadar Hemoglobin Pada Pekerja Indonesia. Diakses Tanggal 07 Juni 2018.

Munawarah, Siti. (2009). Pengaruh Ekstrak Kelopak Rosela (Hibiscus Sabdariffa) Terhadap Peningkatan Kadar Hemoglobin (Hb) Dalam Darah Tikus Putih (Rattus Nurvegicus) Anemia.

Muslihin, A. (2017). Vitamin B1 (Thiamin).https://mediskus.com/nutrisi/ vitaminb1-thiamin. Diakses pada 03 Juni 2018.

Sumarny, R., Rahayu, L., Sandhiutami, N.M.D.,Mory, L., (2013). Efek Stimulansia Infus Lada Hitam Piperis nigri fruktus Pada Mencit. Jurnal Ilmu Kefarmasian Indonesia. 11(2): 142-146. Sudarmi. (1997). Kafein Dalam Pandangan Farmasi. Medan: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universitas Sumatera Utara.

Siregar Yusni Ikhwan dn Adelina. (2009). Pengaruh Vitamin C Terhadap peningkatan (Hb Darah dan Kolulushidupan Benih Ikan Krapu Bebek (Cromileptes Altivelis). Fakults Peikanan dan Ilmu Kelautan. Universitas Riau. Pekan Baru.

Thomas, A.N.S. (1992). Tanaman Obat Tradisional , Edisi 2. Yogyakarta : Kanisius.

Thallon, C. (2010). 100% Organic Red Food Blend.http://www.organicfoodee.com diakses pada tanggal 07 Juni 2018.

Wirakusumah. (2007). Jus Buah dan Sayuran. Jakarta : Swadaya.


Full Text: PDF

DOI: 10.33751/jsi.v7i2.11303

Refbacks

  • There are currently no refbacks.