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Occurrence of Pestalotiopsis sp. Causing Leaf Spot on Eucalyptus pellita under Special Forest Management

Silvia Endrawati, Tri Saptari Haryani, Neo Endra Lelana

Abstract


Leaf spot is one of the most common diseases found on Eucalyptus pellita leaves. This study aimed to isolate and identify the fungal pathogen associated with leaf spot, both morphologically and molecularly. Symptoms observed on diseased leaves included blackish-brown spots, with affected tissues turning yellow to brownish. Morphological identification provided insights into the macroscopic and microscopic characteristics, enabling classification of the fungal isolates to the genus level. In contrast, molecular identification using the Internal Transcribed Spacer (ITS)-5.8S rDNA region allowed determination of the species level. The findings confirmed that Pestalotiopsis sp. was responsible for leaf spot disease on Eucalyptus pellita, as evidenced by morphological and microscopic features. The fungal conidia were characterized by having four septa. Furthermore, alignment with the GenBank database using the BLAST (Basic Local Alignment Search Tool) program validated the molecular identification, and phylogenetic analysis based on ITS fragments supported the classification. These results provide a comprehensive understanding of Pestalotiopsis as the causal agent of leaf spot disease in Eucalyptus pellita and highlight the importance of combining morphological and molecular approaches for accurate pathogen identification.

Keywords


eucalyptus pellita; morphology; molecular; leaf spot disease

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DOI: 10.33751/jsi.v8i1.12814

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