AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)
Abstract
Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.
Keywords
References
Budiarti, Mellia dan Rina Sri Kasiamdari. (2014). Pengaruh Modifikasi Media Budaya Jamur Tiram (pleurotus Ostreatus Jacq. Ex Fr. Kummer) Terhadap Morfologi, Pertumbuhan, dan Kandungan Protein. Skripsi. Universitas Gadjah Mada : Yogyakarta
Chow ST. WW Chaw and Chung. (2003) Antiokxidant activity and safety of 50 %
ethanolic red bean extract (Phascolus raditus L Var Aurea). Journal of Food
Science. Vol. 68(1); 21-25
Depkes RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan RI. hal 5-11.
Febriani D, Dina M dan Endah R. (2015). Karakteristik Simplisia Dan Ekstrak Etanol 70 % Daun Sirsak (Annona muricata Linn). Prosiding Penelitian SPeSIA Unisba : ISSN 2460-6472.
Gagola, C et al. (2004). Aktivitas Antioksidan dari Ekstrak Fenolik Cortex Umbi kayu (Manihot esculenta) Daging putih dan daging kuning yang diambil dari kota melonguane kabupaten kepulauan talaud. Jurnal ilmiah farmasi pharmacon. Vol 3 (2). ISSN 2302-2493.
Hanani et al. (2015). Identifikasi Senyawa Antioksidan dalam Spon Callyspongia SP dari Kepulauan Seribu. Majalah Ilmu Kefarmasian II. (3), Halaman 130.
Huang, D and Prior R.L. 2005. The Chemistry Behind Antioxidan Capacity Assays. Journal Of Agricultural And Food Chemistry. 53:1841-1856.
Jaelani. (2008). Jamur Berkhasiat Obat. Jakarta: Pustaka Obor Populer. Hal: 61-70.
Kristianingsih. (2005). Isolasi dan Identifikasi Senyawa Terpenoid dari Akar Tanaman Kedondong. Skripsi. Institut Pertanian Bogor.
Menkes. (1994). Persyaratan Obat tradisional. Jakarta : Mentri kesehatan.
Middleton E, C Kandaswami & TC Theoharides. (2000). The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacological Reviews. 52. 673 751.
Prakash, A., Rigelhof, F., Miller, E. (2001). Antioxidant Activity. Journal Medalliaon Laboratories Analitycal Progress: Vol 10(2).
Robinson, T., (1995). Kandungan organik Tumbuhan Tinggi, Edisi VI, Hal. 191-216, Diterjemahkan oleh Kosasih Padnawinata, ITB, Bandung.
Sasikumar, J. M., U. Jinu and R. Shamna. (2009). Antioxidant Activity and HPTLC Analysis of Pandanus odoratissimus L. Root. European Journal of Biological Sciences Vol. 1(2): 17 22.
Winarno F.G. (2004). Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama.
DOI: 10.33751/ekol.v19i2.1647
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
