UJI ANTIBAKTERI EKSTRAK DAUN SAMBUNG NYAWA (Gynura procumbens (Lour.) Merr.) DAN DAUN TAPAK LIMAN (Elephantopus scaber L.) TERHADAP Salmonella thypi

Prasetyorini Djarot, Anggita Rahmadini, Novi Fajar Utami

Abstract


This research was aimed to create aromatic candle, infused with cinnamon (Cinnamomum burmanii) bark essential oil, which is a potential repellant for house fly (Musca domestica).  The distillation of akar wangi samples was carried out at the Spice and Aromatic Research Unit by vapor distillation method for 9-12 hours. The aromatic candles were based on a mixture of solid paraffin and stearin in a 2:8 proportion, respectively, enriched with fragrant root bark essential oils with 5 formulas, namely F1 (placebo), F2 (fixative), F3 (1% essential oil), F4 (2% essential oil), and F5 (3% essential oil).  The repellency test was carried out by inserting 20 house flies in a of 50x50x50 cm container that contains a lighted aromatic candle according to the doses mentioned above, and shrimp scalps located 15 cm from the candle that functions as a bait. The calculated parameters were the hourly number of flies that were allured to the bait for 6 hours, which were related to the candles repellency and the house flys preference of candle. The repulsion ability result were 89.724%, 75.28%, 68.06%, 49.17%, 9.72% for F5, F4, F3, F2, and F1, respectively. The result also showed that the F4 sample was the most preferred sample.


Keywords


Gynura procumbens, Elephantopus scaber L, Salmonella thypi, antibacteri, leaf 96% etanol extract

References


, M., Mahdi, N., dan Zuraidah. (2016). Uji Aktivitas Antibakteri Ekstrak Daun Mahkota Dewa Phaleria macrocarpa Terhadap Bakteri Staphylococcus aureus. Prosiding Seminar Nasional Biotik.

Azter, A.A. (2009). Uji Efek Ekstrak Etanol Herba Tapak Liman (Elephantopus scaber L.) Terhadap Penurunan Kadar Asam Urat Darah Pada Tikus Putih Jantan Yang Diinduksi Kafein. Skripsi. Program Studi Farmasi. Fakultas Kedokteran Dan Ilmu Kesehatan. Universitas Islam Negeri (UIN) Syarif Hidayatullah. Jakarta.

Bakhtar, D.D.A., Jubahar, J., Yusdi, E. (2018). Uji Aktivitas Fraksi Dari Ekstrak Daun Sambung Nyawa (Gynura procumbens (Lour)Merr.) Terhadap Bakteri Shigella dysenteriae. Jurnal Farmasi Higea, Vol. 10, No. 1.

Dharma, S., Adirman, dan Elisma. (2013). Efek Analgetik Ekstrak Etanol Daun Tapak Liman (Elephantopus scaber L.) Pada Mencit Putih Jantan. Jurnal Farmasi Higea, Vol. 5, No. 1.

DepKes RI. (1977). Material Medika Indonesia Jilid I. Departemen Kesehatan RI. Jakarta.

_________. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat Cetakan Pertama. Departemen Kesehatan RI. Jakarta.

_________. (2008). Farmakope Herbal Indonesia Edisi I. Jakarta.

Fadli, M.Y. (2015). Benefits of Sambung Nyawa (Gynura procumbens) Subtance as Anticancer. Faculty of Medicine. Lampung University. J Majority. 4 (5).

Lau, S.H.A., Wahyudin, E., Lallo, S. (2018). Potensi Antioksidan Ekstrak Etanol Daun Sambung Nyawa (Gynura procumbens) Terenkapsulasi Maltodextrin dan Pengaruhnya Terhadap Kadar MDA Darah Tikus Wistar Jantan yang Diinduksi CCl4. Majalah Farmasi dan Farmakologi; 22(3):93-98.

Marhamah. (2010). Evalebari Penggunaan Antibiotik Pada Pasien Demam Tifoid Dewasa di Instalasi Rawat Inap RS Umum Daerah Pambalah Batung Kabupaten Hulu Sungai Utara Kalimantan Selatan 2009. Skripsi. Fakultas Farmasi. Universitas Muhamadiyah Surakarta. Surakarta.

Menteri Kesehatan Republik Indonesia. (2006). Keputusan Menteri Kesehatan Republik Indonesia Nomor 364/MENKES/SK/V/2006 Tentang Pedoman Pengendalian Demam Tifoid. Jakarta.

Miranti, M., Prasetyorini, dan Suwarg, C. (2013). Perbandingan Aktivitas Antibakteri Ekstrak Etanol 30% dan 96% Kelopak Bunga Rosella (Hibiscus sabdariffa L.) terhadap bakteri Staphylococcus aureus. Ekologia. Vol. 13 No. 1.

Mohsina Mukti, Asma Ahmed, Shuvro Chowdhury, Zubaida Khatun, 3 Piplu Bhuiyan, 3 Kallol Debnath, 3 Mohammed Rahmatullah. (2012). Medicinal plant formulations of Kavirajes in several areas of Faridpur and Rajbari districts, Bangladesh. American-Eurasian Journal of Sustainable Agriculture, 6(4): 234-247

Nonci, F.Y., Rusli, Atqiyah, A. (2014). Antimikroba Ekstrak Etanol Daun Tapak Liman (Elephantopus scaber L.) Dengan Menggunakan Metode KLT Bioautografi. JF FIK UINAM. 2(4).

Pratama N.H. L. and Cucunawangsih. (2017). Antimicrobial Resistance of Salmonella enterica Serovars typhi and Paratyphi Isolates from a General Hospital in Karawaci. International journal of Microbiology Volume 2017.

F.A., Haniastuti, T., Utami, T.W. (2016). Skrining Fitokimia Dan Antibakteri Ekstrak Etanol Daun Sirsak (Annona muricata L.) Pada Streptococcus mutans ATCC 35668. Majalah Kedokteran Gigi Indonesia. 3(1).

F. M. Rahman and. Sharif Al Asad. (2013). Chemical and biological investigations of the leaves of Gynura procumbens,. Int J of Bio (IJB). 3(4).

Rini, E.P., dan Nugraheni, E.R. (2018). Uji Daya Hambat Berbagai Merk Hand Sanitizer Gel Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Journal of Pharmaceutical Science and Clinical Research.

Sidabutar, S., dan Satari, H.I. (2010). Pilihan Terapi Empiris Demam Tifoid Pada Anak: Kloramfenikol atau Seftriakson?. Sari Pediatri. 11(6).

Simarmata, R., Lekatompessy, S., dan Sukiman, H. (2007). Isolasi Mikroba Endofitik Dari Tanaman Obat Sambung Nyawa (Gynura procumbens) Dan Analisis Potensinya Sebagai Antimikroba. Berk. Panel. Hayati: 13.

Shryock, T.R. and NCCLS, (2002). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals: approved standard. 2nd Edn., pp: 86

Tewari R, Saltanat J. and Mridu D. (2015). Antimicrobial resistance pattern of Salmonella enterica servars in Southern Delhi. International Journal of Community Medicine and Publik Health. 2(3).

Tiwari P. Ritesh Jain, Kuldeep Kumar, Rajnikant Panik, Pratap Kumar Sahu. (2011). An evaluation Of Antimicrobial Activities Of Root Extrac Of Calendula officinalis (Linn). Pharmacologyonline 2:886-892

Trisharyanti D.K., Ika, dan Febriani, R. (2017). Skrining Aktivitas Antibakteri Ekstrak Etanol Daun Terhadap Salmonella thypi Resistensi Kloramfenikol. Journal of Pharmaceutical Science and Clinical Research.

Ugboko H. and Nandita De. (2014). Mechanisms of Antibiotic resistance in Salmonella typhi. Int.J.Curr.Microbiol.App.Sci. 3(12): 461-476

Voight, Rudolf. (1994). Buku Pelajaran Teknologi Farmasi. Gajah Mada University Press. Yogyakarta.


Full Text: PDF

DOI: 10.33751/ekol.v19i1.1662

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.