1. Home
  2. Vol 24, No 1 (2024)
  3. Khaerani

UJI ANTAGONIS Trichoderma sp. TERHADAP FUNGI PATOGEN Pestalotiopsis sp. PENYEBAB PENYAKIT HAWAR DAUN PADA TANAMAN Eucalyptus

Nuraliefah Khaerani, Tri Saptari Haryani, Neo Endra Lelana

Abstract


Leaf blight disease has become one of the significant ailments in Eucalyptus spp. forest plants. This disease can lead to plant growth disruption and even plant mortality. This research aims to evaluate the antagonistic abilities of several Trichoderma isolates against Pestalotiopsis fungi associated with Eucalyptus leaf blight disease. The research was conducted using the dual culture method, wherein both Trichoderma isolates and the pathogen were cultivated on the same petri dish. The obtained data were analyzed using ANOVA at a 5% significance level, followed by the Duncan test in case of significant differences. The results of BLAST analysis of pathogens causing leaf blight have the highest similarity with Pestalotiopsis uvicola, which is equal to 99.82%. The Results test antagonist show percentage inhibition Trichoderma to pathogen Pestalotiopsis biggest showed by isolate TL122 ie of 65.333%, followed with isolates TL411 and TL631 ie consecutive by 54.49% and 49.33%. The T.L.122 isolate was declared to be the most effective as a potential antagonist fungus against the Pestalotiopsis pathogen which causes leaf blight in Eucalyptus plants.

Keywords


Dual culture, inhibition, Pestalotiopsis uvicola.

References


Alfizar, Marlina, Susanti, F. (2013). Kemampuan Antagonis Trichoderma Terhadap Beberapa Fungi Patogen In Vitro. Prodi Agroteknologi Fakultas Pertanian, Universitas Syiah Kuala, Darussalam.

Amaria, W., Soesanthy, F., Ferry, Y. (2016). Keefektifan Biofungisida Trichoderma sp. Dengan Tiga Jenis Bahan Pembawa Terhadap Jamur Akar Putih Rigidoporus Microporus. J. TIDP 3(1), 37–44. Balai Penelitian Tanaman Industri dan Penyegar.

Carvalho, D.D.C., Oliveira, R.M., Marques, M.G. (2019). Molecular, morphophysiological and pathogenic characterization of eucalypt Pestalotiopsis grandis-urophylla isolates, a new species. Trop. plant pathol. 44, 132–139. https://doi.org/10.1007/s40858-019-00277-0

Cornejo, H.A.C. Iguez, L.M.ias, Val. E. del, & Larsen, J. (2016). Fungsi ekologis Trichoderma sp. Ekologi Mikrobiologi, 92(1), 1-17

Dendang, B. (2015). Uji Antagonisme Trichoderma spp. terhadap Ganoderma sp. yang Menyerang Tanaman Sengon Secara in-vitro. Penelitian Kehutanan Wallace, Vol 4 (2).

El-Debaiky, SA. (2017). Antagonistic studies and hyphal interactions of the new antagonist Aspergillus piperis against some phytopathogenic fungi in vitro in comparison with Trichoderma harzianum. Microbial Pathogenesis, Vol. 113 Pages 135-143. Tanta University, Egypt.

Herliyana EN., Jamilah R, Taniwiryono D, Firmansyah MA. (2013). Uji In-vitro Pengendalian Hayati oleh Trichoderma spp. terhadap Ganoderma yang Menyerang Sengon. Silvikulltur Tropika, Vol 4 (3)

Hidayat, N., Rajab, A., Mudi, L. (2021). Uji Invitro Daya Hambat Fungi Endofit Asal Tanaman Rambusa (Passiflora foetida) Sebagai Agens Pengendali Hayati Penyakit Layu Fusarium. Jurnal Agrotech, Vol 11 (2) 64-70. Politeknik Pertanian Negeri Samarinda.

Huda N, Imaningsih W, Hakim SS. (2019). Uji Antagonisme Fungi Endofit Tanaman Galam (Melaleuca cajuputi) terhadap Calonectria truncatum. Jurnal Mikologi Indonesia, Vol 3 No (2). Universitas Lambung Mangkurat, Banjarbaru, Kalimantan Selatan.

Ismail, N., & Andi Tenrirawe. (2011). Potensi Agen Hayati Trichoderma harzianum Sebagai Agens Pengendali Hayati. Seminar Regional Inovasi Teknologi Pertanian. Sulawesi Utara.

Izzatinnisa’, Utami U, Mujahidin A. (2020). Uji Antagonisme Beberapa Fungi Endofit pada Tanaman Kentang terhadap Fusarium oxysporum secara In Vitro. Jurnal Riset Biologi dan Aplikasinya, Vol 2 (1). Universitas Islam Negeri Malang.

Jia M, Chen L, Xin HL, Zheng, C-J. Han T, Qin LP. (2016). Friendly Relationship between Endophytic Fungi and Medicinal Plants: A Systematic Review. Frontiers in Microbiology (7): 1-4. doi: 10.3389/fmicb.2016.00906

Kohl, J., Kolnaar, R., and Ravensberg, W.J.2019. Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. Frontiers in Plant Science, 10(July): 1-19. https//doi.org/10.3389/fpls.2019.0084559

Kubicek, Christian P. (2019). Evolution and comparative genomics of the most common Trichoderma species. Research Article BMC Genomic. 20(1), 1–24.

Lelana, EN., Anggraeni, I., Mindawati, N. (2015). Uji Antagonis Aspergillus sp. Dan Trichoderma spp. Terhadap Fusarium sp., Penyebab Penyakit Rebah Kecambah Pada Sengon. Jurnal Penelitian Hutan Tanaman Vol. 12 No. 1, 23-28.

Maimunah, RAK. Juni 2013. Hama Tanaman Pertanian. Agricultural Crop Pests, Buku Ajar, No. 4. Universitas Medan Area.

Márquez, S. S., Bills, G. F., and Zabalgogeazcoa, I. (2011). Fungal species diversity in juvenile and adult leaves of Eucalyptus globulus from plantations affected by Mycosphaerella leaf disease. Ann. Appl. Biol. 158, 177–187. doi: 10.1111/j.1744-7348. 2010.00449. x

Morales-Rodríguez, C., Dalla Valle, M., Aleandri, M.P. & Vannini, A. (2019). Pestalotiopsis biciliata, a new leaf pathogen of Eucalyptus, spp. recorded in Italy. Forest Pathology. 49: 1–7. DOI:10.1111/efp.12492.

Muhibbudin, A., Setiyowati, EM., Sektiono, AW. (2021). Mechanism Antagonism Of Trichoderma Viride Against Several Types Of Pathogens And Production Of Secondary Metabolites. Universitas Brawijaya. Malang. Journal of Agricultural Sciences, Vol. 4 (1).

Muksin, R., Rosmini, Panggeso, J. (2013). Uji Antagonisme Trichoderma Terhadap Fungi Patogen Alternaria porri Penyebab Penyakit Bercak Ungu Pada Bawang Merah Secara In-Vitro. Agrotekbis, Vol 1 (2). Untad.

Ristiari, NPN., Julyasih, KSM., Suryanti, KAP. (2018). Isolasi dan Identifikasi Fungi Mikroskopis pada Rizosfer Tanaman Jeruk Siam (Citrus nobilis lour.) Dikecamatan Kintamani, Bali. Jurnal Pendidikan Biologi Undiksha, Vol. 6 (1). Universitas Pendidikan Ganesha. Singaraja.

Sabo, VA and Knezevic, V. (2019). Antimicrobial activity of Eucalyptus camaldulensis Dehn. plant extracts and essential oils: A review. Industrial Crops & Products, Vol.132 (413–429). University of Novi Sad, Trg Dositeja Obradovica 2, Novi Sad, Vojvodina, Serbia.

Siallagan, BR., Anna, N., Siregar, EBM. (2015). Uji Infeksi Cylindrocladium sp., Terhadap Klon Hibrida Turunan Eucalyptus grandis x Eucalyptus urophylla. Peronema Forestry Science, Vol 4 (3). Universitas Sumatera Utara. Medan.

Suwannarach N, Kumla J, Bussaban B & Lumyong S. (2012). New report of leaf blight disease on eucalyptus (Eucalyptus camaldulensis) caused by Pestalotiopsis virgatula in Thailand, Canadian Journal of Plant Pathology, 34:2, 306-309, DOI: 10.1080/07060661.2012.680501.

White, TJ., Bruns, T., Lee, S., Taylor, JW. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, MA., Gelfand, DH., Sninsky, JJ., White, TJ., editor. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press Inc; 1990. Pp. 315–322.

Widyaningsih, S and Triasih, U. (2020). Biological Control of Strawberry Crown Rot Disease (Pestalotiopsis sp.) using Trichoderma harzianum and Endophytic Bacteria. IOP Conf. Series: Earth and Environmental Science 752 (2021) 012052.

Zivkovic, S., Stojanovic, S., Ivanovic, Z., Gavrilovic, V., Popovic, T., & Balaz, J. (2010). Screening of Antagonistic Ativity of Microorganism Against Colletotrichum acutatum and Colletotrichum gloeosporoides. Arch. Boil. Sci. Belgrade, (3): 611-62.


Full Text: PDF

DOI: 10.33751/ekologia.v24i1.9149

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.