Effectiveness of Citronella Extract (Cymbopogon nardus) Mouthwash From Microwave-Assisted Extraction Method Against Streptococcus mutans

Oom Komala; Siti Mahyuni; Anna Pradiningsih; Elfrieda Kusuma Dewi

doi:10.33751/jf.v14i1.9621

Effectiveness of Citronella Extract (Cymbopogon nardus) Mouthwash From Microwave-Assisted Extraction Method Against Streptococcus mutans

Oom Komala, Siti Mahyuni, Anna Pradiningsih, Elfrieda Kusuma Dewi

Abstract

Cymbopogon nardus, or citronella has a distinctive aroma is known as a source of essential oils. Extract of citronella can be use as mouthwash to reduce bad breath and protect teeth from bacteria that cause dental caries. C. nardus contains antibacterial compounds such as flavonoid, essential oils, polyphenols, and saponin which are also act as antioxidant compounds. This research was carried out to formulate mouthwash using citronella extract and determine the antibacterial activity of the citronella mouthwash against S. mutans bacteria using disc diffusion method. The microwave extraction method (MAE) was used to obtain extract using 70 % and 96 % ethanol as a solvents. The mouthwash were made in 3 formulas i.e. formula 1, formula 2, and formula 3 each contain 25 % w/v, 30 % w/v, and 35 % w/v) respectively. The quality of mouthwash includes organoleptic, pH, viscosity and hedonic was evaluated and antibacterial activity was determined by measuring the value of minimum inhibitory concentration (MIC) and diameter of inhibition zone. The MIC value of citronella extract was achieved at concentration 20 %. All mouthwash formula met the requirements for a good mouthwash. Formula 1 was the most preferred formula by the panelists based on the hedonic test. Formula 3 had the strongest antibacterial activity with diameter inhibitory of 17.6 ± 0.52 mm. It was concluded that citronella mothwash has the potential to be developed into an antibacterial mouthwash.

Keywords

Citronella; Cymbopogon nardus; MAE; Mouthwash; Streptococcus mutans

References

Alara, O.R., Abdurahman, N.H., Olalere, O.A. (2018). Ethanolic extraction of bioactive compounds from Vernonia amygdalina leaf using response surface methodology as an optimization tool. Journal of Food Measurement and Characterization, 12, 1107-1122. doi: 10.1007/s11694-018-9726-3.

Alfiyanti, W. I., FerdyantiA. P., RohmanA. F., Agustina E., Putnamasari, R., & KusumawatiE. (2023). Phtochemical Compounds Test of Lemongrass Extract (Cymbopogon Citratus) Using Maceration Method. International Conference on Sustainable Health Promotion, 3(1), 30-42.

Astuti, S. (2013). Skrining Fitokimia dan Uji Aktivitas Antibiotika Ekstrak Etanol Daun, Batang, Bunga dan Umbi Tanaman Binahong (Andredera cordifolia (Ten) Steenis). Journal of Chemical Information and Modeling, 19.

Bassolé, I. H. N., Lamien-Meda, A., Bayala, B., Obame, L.C., Ilboudo, A. J., Franz, C., Novak, J., Nebié, R. C., & Dicko, M. H. (2011). Chemical Composition and Antimicrobial Activity of Cymbopogon citratus and Cymbopogon giganteus Essential Oils Alone and in Combination. Jurnal Phytomedicine Elsevier, 18(12), 1070-1074. https://doi.org/10.1016/j.phymed.2011.05.009

BPOM RI. (2015). Cairan Kumur. Pusat Informasi Obat Nasional: Badan Pengawas Obat Dan Makanan Republik Indonesia.

Depkes RI (2000). Informasi Obat Nasional Indonesia. Ministry of Health of the Republic of Indonesia. Jakarta.

Depkes RI. (2008). Profil Kesehatan Indonesia 2007. Republic of Indonesia Health Department, Jakarta

Hanani, E. (2015). Analisis Fitokimia. Penerbit Buku Kedokteran EGC. Jakarta.

Hasibuan, S. Y., Amallia, C., Hutagalung, M. H., & Erawati, S. (2021). Perbandingan Efektivitas Ekstrak Sereh dengan Temulawak dalam Menghambat Pertumbuhan Streptococcus Mutans. Jurnal Ilmiah Kesehatan Sandi Husada, 10(1), 208-213.

Hudzicki, J. (2009). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. American Society for Microbiology, pp: 1-24.

Justicia, A. K., Ferdinan, A., dan Maya, M. (2017). Formulasi Mouthwash Minyak Atsiri Daun Kemangi (Ocimumsanctum L.) dan Kayu Manis (Cinnamomum zeylanicum) dengan Menggunakan Tween 80 Sebagai Surfaktan. Jurnal Ilmiah Ibnu Sina, 2(1): 134-146.

Lemos, J.A., Palmer, S.R., Zeng, L., Wen, Z.T., Kajfasz, J.K., Freires, I.A., Abranches, J., Brady, L.J. (2019). The Biology of Streptococcus mutans. Microbiol Spectr. 7(1):10.1128 doi:10.1128/microbiolspec.GPP3-0051-2018.

Noval, N., Melviani, M., Novia, N., & Syahrina, D. (2020). Formulasi Dan Evaluasi Sediaan Obat Kumur (Mouthwash) Dari Ekstrak Etanol Tanaman Bundung (Actinoscirpus Grossus) Sebagai Antiseptik Mulut. Jurnal Surya Medika 6(1):112-120

esearchgate.net/publication/345199263_Formulasi_Dan_Evaluasi_Sediaan_Obat_Kumur_Mouthwash_Dari_Ekstrak_Etanol_Tanaman_Bundung_Actinoscirpus_Grossus_Sebagai_Antiseptik_Mulut

Quan, P.T., Tong V. H., Nguyen H. H., Nguyen X. D., Troung N.T. (2006). Micrawave-Assisted Extraction Of Polyphenols From Fresh Tea Shoot. Science & Technologi development, 9(8). 69-75.

Rathee, M. & Sapra, A. (2023). Dental Caries. StatPearls [Internet]. National Library of Medicine. StatPearls Publishing.

https://www.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg

Rasmah., Dwyana, Z., Tambaru, E., Rante, H. (2016). Uji aktivitas Sediaan Obat Kumur Ekstrak Daun Miana Coleus scutellarioides (L) Benth terhadap Pertumbuhan Jamur Candida albicans. Jurnal Penelitian Fakultas Farmasi Universitas Hassanuddin.

Reveny, J. (2011). Daya Antimikroba Ekstrak dan Fraksi Daun Sirih Merah (Piper betle Linn.). Jurnal ILMU DASAR, 12(1), 6-12.

Routray, W., and Orsat, V. (2012). Microwave-Assisted Extraction of Flavonoid: A Review. Food Bioprocess Technology 5, 409-424. doi: 10.1007/s11947-011-0573- z.

Rowe, C.R., Sheskey, J. P., dan Quin, E. M. (2009). Handbook of Pharmaceutical Excipient. Sixth Ed. Washington: Pharmaceutical Press. Pp. 605.

Shakinaz, W., A.E.S., Refaat, A.A., dan El, S.S.T. (2010). Production of Biodiedel Microwaves Technique, J. Advanced Reasearch, 1, 309-314.

Stenudd, C, Nordlund A, Ryberg M, et al.. The association of bacterial adhesion with dental caries.. J. Dent Res. 2001;80: 2005-2010.

Tampoliu, M. K. K., Ratu, A. P., & Rustiyaningsih, R. (2021). formula dan aktivitas antibakteri obat kumur ekstrak batang serai WANGI (Cymbopogon nardus L.) Terhadap Bakteri Streptococcus mutans. Jurnal Kesehatan Poltekkes Palembang (JPP), 16(1), 29-39.

Wijayanti, R., Rosyid, A. (2015). Efek Ekstrak Kulit Umbi Bawang Putih (Allium sativum L.) Terhadap Penurunan Kadar Glukosa Darah Pada Tikus Putih Jantan Galur Wistar Yang Diinduksi Aloksan. Journal of Pharmaceutical Science & Clinical Pharmacy. 12(1). 47-52.

Zhao, J., Fan, Y., Cheng, Z., Kennelly, E.J., & Long, C. (2024). Ethnobotanical uses, phytochemistry and bioactivities of Cymbopogon plants: A review. Journal of Ethnopharmacology. Volume 330, 118181.

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DOI: 10.33751/jf.v14i1.9621